Separation of Bile Acids by Paper Chromatography. Bile Acids and Steroids. 3.

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Bile Acids and Steroids

In several mammalian species, i.e. rabbit, dog and man, deoxycholic acid is one of the main bile acids in the bile (1). In recent years evidence has accumulated that deoxycholic acid is formed from cholic acid during the enterohepatic circulation by the action of intestinal microorganisms. By the use of 24-@-labeled cholic acid this conversion has been demonstrated in rabbit and in man (2,3). B...

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1. A method is described for the quantitative isolation of bile acids from cellular material. Homogenates of rat liver are freeze-dried and extracted exhaustively with 95% (v/v) ethanol containing 0.1% (v/v) of aq. ammonia (sp.gr. 0.88) and purified by anion-exchange chromatography on Amberlyst A-26. 2. The extracted bile acid conjugates are subjected to either of two hydrolytic procedures, one...

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Steroids Derived from Bile Acids

Derivatives of bile acids with substituents in Ring C have been described in the preceding papers of this series (l-6). It is the purpose of this paper to assign the probable stereochemical configurations for these compounds. As desoxycholic acid (I)l is a convenient reference compound, it will first be necessary to discuss briefly the spatial arrangement of certain portions of this bile acid. ...

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Chromatography is a common technique for separating chemical substances. The prefix “chroma,” which suggests “color,” comes from the fact that some of the earliest applications of chromatography were to separate components of the green pigment, chlorophyll. You may have already used this method to separate the colored components in ink. In this experiment you will use chromatography to separate...

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ژورنال

عنوان ژورنال: Acta Chemica Scandinavica

سال: 1952

ISSN: 0904-213X

DOI: 10.3891/acta.chem.scand.06-1552